{"product_id":"anti-flag-tag-dykddddk-antibody-2h8-bha21409453","title":"Anti-Flag Tag (DYKDDDDK) Antibody (2H8)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Flag Tag (DYKDDDDK) Antibody (2H8) is intended for detection of \u003cstrong\u003eFlag Tag\u003c\/strong\u003e. Commonly used in CHIP, IF, IP, WB applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Validate antibody specificity by WB with KD\/KO control lysates, Detect target localization by IF\/ICC in cultured cells, Enrich target by IP\/Co-IP from cell lysates for downstream analysis, Map DNA binding by ChIP-qPCR in crosslinked chromatin, Optimize antibody dilution for staining in cells\/tissues using a reference sample. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Flag peptide, Flag-tag.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a, kappa; Purification: Protein A\/G purified from cell culture supernatant.; Format: Liquid.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eFlag Tag (Flag peptide) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular \u0026amp; Cellular Biology. Common alternative names and symbols used in the literature include: Flag-tag. Based on the annotated name\/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, include fresh protease inhibitors (and phosphatase inhibitors for PTM targets) and verify the dominant band at the expected size; additional bands may reflect isoforms, processing, or cross-reactivity.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037061472621,"sku":"YP666023-50UG","price":128.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037790822765,"sku":"YP666023-100UG","price":218.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YP666023-SDSPAGE-1.jpg?v=1771748285","url":"https:\/\/www.ebiohippo.com\/products\/anti-flag-tag-dykddddk-antibody-2h8-bha21409453","provider":"BioHippo","version":"1.0","type":"link"}