{"product_id":"anti-gad65-gad2-antibody-picoband-bha21006771","title":"Anti-GAD65\/GAD2 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-GAD65\/GAD2 Antibody Picoband® is an antibody targeting \u003cstrong\u003eGAD2\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, Flow Cytometry, ELISA\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eMouse,Rat\u003c\/strong\u003e; observed MW: \u003cstrong\u003e65 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e65411 MW\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-GAD65\/GAD2 Antibody catalog # PA1403. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GAD2 — Glutamate decarboxylase 2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Mouse,Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 65 kDa; Calculated: 65411 MW\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e Catalyzes the production of GABA.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Cytoplasm, cytosol . Cytoplasmic vesicle . Cell junction, synapse, presynaptic cell membrane ; Lipid-anchor . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details (datasheet):\u003c\/strong\u003e Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Belongs to the group II decarboxylase family.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Metabolic Signaling Pathways,Metabolism,Neurology Process,Neuroscience,Neurotransmitter,Pathways and Processes.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069788512621,"sku":"PA1403","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070113309037,"sku":"PA1403-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070113341805,"sku":"PA1403-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070113374573,"sku":"PA1403-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070113407341,"sku":"PA1403-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070113440109,"sku":"PA1403-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070113472877,"sku":"PA1403-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070113505645,"sku":"PA1403-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070113538413,"sku":"PA1403-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070113571181,"sku":"PA1403-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070113603949,"sku":"PA1403-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070113636717,"sku":"PA1403-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pa1403-gad2-primary-antibodies-wb-testing-1.jpg?v=1772612546","url":"https:\/\/www.ebiohippo.com\/products\/anti-gad65-gad2-antibody-picoband-bha21006771","provider":"BioHippo","version":"1.0","type":"link"}