{"product_id":"anti-gcn4-nanobody-saa1016-bha21409473","title":"Anti-GCN4 Nanobody (SAA1016)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-GCN4 Nanobody (SAA1016) is intended for detection of \u003cstrong\u003eGCN4 Nanobody\u003c\/strong\u003e. Commonly used in ELISA applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Develop ELISA pairing using capture\/detection antibodies and a standard curve, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e General control transcription factor GCN4, Amino acid biosynthesis regulatory protein, General control protein GCN4, YEL009C, AAS101, GCN4, AAS3, ARG9.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Alpaca; Clonality: Monoclonal; Isotype: VHH-8His-Cys-tag; Purification: Purified by Nickel column.; Format: Liquid; Reactivity: Saccharomyces cerevisiae (strain ATCC 204508 \/ S288c) (Baker's yeast).\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eGCN4 Nanobody (General control transcription factor GCN4) is annotated as a DNA-binding transcriptional regulator that influences gene-expression programs. Such regulators often show context-dependent expression and nuclear localization. Common alternative names and symbols used in the literature include: Amino acid biosynthesis regulatory protein, General control protein GCN4, YEL009C, AAS101, GCN4, AAS3, ARG9. Based on the annotated name\/class, the antigen is most often associated with nucleus localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037062226285,"sku":"JN839023-50UG","price":221.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037791510893,"sku":"JN839023-100UG","price":372.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-gcn4-nanobody-saa1016-bha21409473","provider":"BioHippo","version":"1.0","type":"link"}