{"product_id":"anti-gnaq-rabbit-monoclonal-antibody-bha21010564","title":"Anti-GNAQ Rabbit Monoclonal Antibody","description":"\u003cp\u003eBoster Bio Anti-GNAQ Rabbit Monoclonal Antibody catalog # M00898-1. Tested in IHC, WB application. This antibody reacts with Human, Mouse, Rat.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProtein\u003c\/strong\u003e: Glycogen synthase kinase-3 beta (gsk3b) | \u003cstrong\u003eGene\u003c\/strong\u003e: Protein lin-28 homolog B | \u003cstrong\u003eTarget\u003c\/strong\u003e: GNAQ | \u003cstrong\u003eFunction\u003c\/strong\u003e: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11\/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11- independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. . | \u003cstrong\u003eResearch areas\u003c\/strong\u003e: Cancer,Epigenetics and Nuclear Signaling,Tumor Biomarkers\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e: Rabbit | \u003cstrong\u003eClonality\/Clone\u003c\/strong\u003e: Monoclonal \/ Clone: 30G74 | \u003cstrong\u003eIsotype\u003c\/strong\u003e: IgG | \u003cstrong\u003eReactivity\u003c\/strong\u003e: Human,Mouse,Rat | \u003cstrong\u003eImmunogen\u003c\/strong\u003e: A synthesized peptide derived from human GNAQ | \u003cstrong\u003eConcentration\u003c\/strong\u003e: 0.5mg\/ml | \u003cstrong\u003ePurification\u003c\/strong\u003e: Affinity-chromatography\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e: WB, IHC, IF, ICC\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eObserved MW\u003c\/strong\u003e: 42 kDa | \u003cstrong\u003eCalculated MW\u003c\/strong\u003e: 27084 MW\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization\u003c\/strong\u003e: Nucleus. Nucleus, nucleolus . Cytoplasm . Predominantly nucleolar (PubMed:22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064). .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details\u003c\/strong\u003e: Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463). .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStatus\u003c\/strong\u003e: Active\u003c\/p\u003e","brand":"Boster Bio","offers":[{"title":"100 uL\/vial \/ Unconjugated","offer_id":53072133063021,"sku":"M00898-1","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/m00898-1-gnaq-primary-antibodies-wb-testing-1.jpg?v=1772695680","url":"https:\/\/www.ebiohippo.com\/products\/anti-gnaq-rabbit-monoclonal-antibody-bha21010564","provider":"BioHippo","version":"1.0","type":"link"}