{"product_id":"anti-gprc5b-antibody-bha21300491","title":"Anti-GPRC5B Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-GPRC5B Antibody is an antibody targeting G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2 (also reported as G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Intracellular, C-terminus.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Rat - identical; Human - 15 out of 16 amino acid residues identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Unconjugated (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eGPRC5B is a member of the large G-protein-coupled receptor (GPCR) superfamily of receptors that share a common structure of seven membrane-spanning domains, an extracellular N-terminal domain, an intracellular C-terminal domain, and many conserved residues[1].The ligand-binding domain of GPCRs is variable and the GPCRs have been divided into three classes based on the ligands that stimulate them, as well by key sequence motifs conserved within phylogenetically related subfamily members.GPRC5B (also known as Retinoic Acid-Induced Gene 2 Protein (RAIG2)) is an orphan GPCR most closely related to the Class C GPCR family, based on homology. Class C GPCRs also includes the metabotropic glutamate, calcium-sensing receptor, GABAB, and pheromone receptors.Molecular biology approaches and knockout mouse studies reveal that GPRC5 family proteins have pivotal roles in cancer progression and control of metabolic homeostasis pathways[2].GPCR5B is known to be involved in the regulation of brain homeostasis, proteins related to adhesion or signaling, and remarkably, GPRC5B-mediated tyrosine-phosphorylation signaling cascades play a critical role in development of obesity and insulin resistance through dynamic sphingolipid metabolism[3].GPRC5B contains multiple phosphorylated residues in its carboxyl terminus. Phosphorylation of GPRC5b by the tyrosine kinase Fyn and the subsequent direct interaction with the Fyn Src homology 2 (SH2) domain were found to be critical for the initiation and progression of inflammatory signaling in adipose tissue[4].\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eComparing target expression across perturbations, genotypes, or treatment conditions.\u003c\/li\u003e   \u003cli\u003eInterpreting localization shifts alongside pathway or phenotypic readouts.\u003c\/li\u003e   \u003cli\u003eUsing orthogonal controls (KO\/KD, peptide competition, isotype concepts) to support conclusions.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e   \u003cli\u003eImmunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-GR072.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-GR072; Negative control: BLP-GR072.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064831041901,"sku":"AGR-072-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064905064813,"sku":"AGR-072-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064905097581,"sku":"AGR-072-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064905130349,"sku":"AGR-072-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AGR-072_ept.png?v=1772460990","url":"https:\/\/www.ebiohippo.com\/products\/anti-gprc5b-antibody-bha21300491","provider":"BioHippo","version":"1.0","type":"link"}