{"product_id":"anti-gprc5d-anti-cd3-bispecific-antibody-bha18200129","title":"Anti-GPRC5D-Anti-CD3 Bispecific Antibody","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cp class=\"bhp-cat-pill\"\u003eImmunotherapy\u003c\/p\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eAnti-GPRC5D-Anti-CD3 bispecific antibody is a purified recombinant human bispecific antibody with T cell engager. This bispecific antibody has been tested in a functional assay using NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #60621) in the presence of GPRC5D CHO Recombinant Cell Line (BPS Bioscience #78337) that overexpress GPRC5D (G protein-coupled receptor, class C group 5 member D).\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eIgG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eProtein A affinity purification (HEK293)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e≥90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eHeavy Chain: 49 kDa + glycans; Light Chain: 24 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003e8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e1.62 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStable for at least 12 months at -80°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eT Cell Activation Assay\u003c\/li\u003e\n\u003cli\u003eReporter Assay\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAssay conditions:\u003c\/strong\u003e This antibody was tested as followed. GPRC5D CHO cells (#78337) were incubated with NFAT Luciferase Reporter Jurkat cells (#60621) in a 96-well plate at a 1:2 ratio. Anti-GPRC5D-Anti-CD3 Bispecific Antibody and the negative control Anti-Claudin18.2-Anti-CD3 IgG Bispecific Antibody (#101541) were serially diluted and added to the cells. After 20 hours, luciferase activity was measured with ONE-Step™ Assay System (#60690) and luminescence was measured in a plate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUsage note:\u003c\/strong\u003e Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze\/thaw cycles.\u003c\/p\u003e\n\u003ch2\u003eImmunogen \/ Antigen Information\u003c\/h2\u003e\n\u003cp\u003eImmunogen information not provided. Please refer to the product datasheet for detailed antigen information.\u003c\/p\u003e\n\u003ch2\u003eCross-Reactivity \u0026amp; Specificity\u003c\/h2\u003e\n\u003cp\u003eCross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eBiosafety Level:\u003c\/strong\u003e Not applicable (BSL-1)\u003c\/div\u003e\n\u003cp\u003eAvoid freeze\/thaw cycles.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"25 ug","offer_id":53327927050605,"sku":"102450-1","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53327932096877,"sku":"102450-2","price":510.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53327932129645,"sku":"102450-3","price":2500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/102450_copy.png?v=1778728311","url":"https:\/\/www.ebiohippo.com\/products\/anti-gprc5d-anti-cd3-bispecific-antibody-bha18200129","provider":"BioHippo","version":"1.0","type":"link"}