{"product_id":"anti-hax1-antibody-picoband-bha21004390","title":"Anti-HAX1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-HAX1 Antibody Picoband® is an antibody reagent for detection of \u003cstrong\u003eHAX1\u003c\/strong\u003e (cyclin dependent kinase 8). Researchers commonly use anti-HAX1 antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-HAX1 Antibody Picoband® catalog # A01495-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HAX1 — ATP-binding cassette sub-family G member 8 (cyclin dependent kinase 8). Alternative names: Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35; CDK8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Polyclonal; Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies context:\u003c\/strong\u003e Host: Rabbit, Reactivity: Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Immunogen affinity purified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e E.coli-derived human HAX1 recombinant protein (Position: M1-R279).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e observed 36 kDa, calculated 75679 MW (reported)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProvided application(s):\u003c\/strong\u003e WB, IHC, Flow, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThese attributes help contextualize how the antibody is commonly selected (host\/clonality\/isotype\/label) and how signals are interpreted across sample types and assay formats.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eFunction:\u003c\/strong\u003e Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Nucleus.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details:\u003c\/strong\u003e Predominantly expressed in the liver (PubMed:11099417, PubMed:11452359). Low expression levels in the small intestine and colon (PubMed:11099417). Very low levels in other tissues, including brain, heart and spleen (PubMed:11452359).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e HCLS1-associated protein X-1 is a protein that in humans is encoded by the HAX1 gene. The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eQuantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.\u003c\/li\u003e \u003cli\u003eIsoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.\u003c\/li\u003e \u003cli\u003eContext-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e compare relative target abundance and apparent size shifts (e.g., isoforms\/PTMs) across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e assess distribution across tissue compartments and compare staining patterns between groups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and compare shifts after stimulation or differentiation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAcross these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eApparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.\u003c\/li\u003e \u003cli\u003eSpecies reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.\u003c\/li\u003e \u003cli\u003eControl concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO\/KD, peptide competition, or independent assays) to support interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eFor antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProt entry for HAX1 (O00165) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/O00165 - Ensembl gene summary for HAX1 — Ensembl — https:\/\/www.ensembl.org - HGNC gene symbol report for HAX1 — HGNC — https:\/\/www.genenames.org - Antibody validation concepts (general) — NIH\/NCBI resources — https:\/\/www.ncbi.nlm.nih.gov --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53068597330285,"sku":"A01495-2","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53068799115629,"sku":"A01495-2-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53068799148397,"sku":"A01495-2-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53068799181165,"sku":"A01495-2-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53068799213933,"sku":"A01495-2-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53068799246701,"sku":"A01495-2-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53068799279469,"sku":"A01495-2-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53068799312237,"sku":"A01495-2-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53068799345005,"sku":"A01495-2-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53068799377773,"sku":"A01495-2-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53068799410541,"sku":"A01495-2-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53068799443309,"sku":"A01495-2-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01495-2-hax1-primary-antibodies-wb-testing-1.jpg?v=1772611288","url":"https:\/\/www.ebiohippo.com\/products\/anti-hax1-antibody-picoband-bha21004390","provider":"BioHippo","version":"1.0","type":"link"}