{"product_id":"anti-helicobacter-pylori-vaca-antibody-n02-h1-bha21405434","title":"Anti-Helicobacter pylori vacA Antibody (N02-H1)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against Helicobacter pylori vacA (Vacuolating cytotoxin autotransporter) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eVacuolating cytotoxin autotransporter is a transporter that mediates movement of specific solutes across membranes, supporting metabolic homeostasis and cellular signaling. Transporters are frequently profiled in pharmacology, metabolism and tissue physiology studies.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eIntegral membrane protein localized to plasma membrane or organellar membranes depending on transporter class.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., ELISA, FCM, SPR, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Helicobacter pylori (Campylobacter pylori), Helicobacter pylori (strain ATCC 700392 \/ 26695) (Campylobacter pylori)).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of Helicobacter pylori vacA, and a positive control sample known to express Helicobacter pylori vacA.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036927123821,"sku":"JN909053-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037527499117,"sku":"JN909053-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037527531885,"sku":"JN909053-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/JN909053-SDS-PAGE-1.jpg?v=1771745915","url":"https:\/\/www.ebiohippo.com\/products\/anti-helicobacter-pylori-vaca-antibody-n02-h1-bha21405434","provider":"BioHippo","version":"1.0","type":"link"}