{"product_id":"anti-hif1-beta-arnt-antibody-picoband-bha21000053","title":"Anti-HIF1 beta\/ARNT Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003eARNT (Aryl hydrocarbon receptor nuclear translocator)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-HIF1 beta\/ARNT Antibody Picoband® catalog # PB9129. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively. (reported region: V416-E789).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 87 kDa; calculated MW: 86636 MW\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eAryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator. ARNT is also known as HIF1-beta or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. ARNT is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism. Functional note: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1\/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. Reported localization: Nucleus. Expression\/tissue context: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMitochondrial Markers: Researchers commonly examine how ARNT (Aryl hydrocarbon receptor nuclear translocator) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eMitochondrial Metabolism: Researchers commonly examine how ARNT (Aryl hydrocarbon receptor nuclear translocator) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003ePathways and Processes: Researchers commonly examine how ARNT (Aryl hydrocarbon receptor nuclear translocator) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative ARNT (Aryl hydrocarbon receptor nuclear translocator) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFamily \/ similarity context:\u003c\/strong\u003e Contains 1 bHLH (basic helix-loop-helix) domain.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for P27540: https:\/\/www.uniprot.org\/uniprotkb\/P27540\/entry - PubMed search (ARNT): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARNT - NCBI Gene search (ARNT): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARNT - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066816389485,"sku":"PB9129","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53066884972909,"sku":"PB9129-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53066885005677,"sku":"PB9129-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53066885038445,"sku":"PB9129-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53066885071213,"sku":"PB9129-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53066885103981,"sku":"PB9129-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53066885136749,"sku":"PB9129-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53066885169517,"sku":"PB9129-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53066885202285,"sku":"PB9129-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53066885235053,"sku":"PB9129-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53066885267821,"sku":"PB9129-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53066885300589,"sku":"PB9129-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pb9129-1-WB-anti-hif1-beta-picoband-antibody.jpg?v=1772608083","url":"https:\/\/www.ebiohippo.com\/products\/anti-hif1-beta-arnt-antibody-picoband-bha21000053","provider":"BioHippo","version":"1.0","type":"link"}