{"product_id":"anti-hmg4-hmgb3-antibody-picoband-bha21000534","title":"Anti-HMG4\/HMGB3 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003eHMGB3 (High mobility group protein B3)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-HMG4\/HMGB3 Antibody Picoband® catalog # PB9633. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 23 kDa; calculated MW: 22980 MW\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human,Mouse,Rat\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e Flow Cytometry, IF, IHC, ICC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eHigh mobility group protein B3; High mobility group protein B3. High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. Functional note: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non- canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA\/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity). . Reported localization: Nucleus . Chromosome . Cytoplasm . Expression\/tissue context: Expressed predominantly in placenta.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eChromatin Binding Proteins: Researchers commonly examine how HMGB3 (High mobility group protein B3) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eChromosome Structure: Researchers commonly examine how HMGB3 (High mobility group protein B3) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eDNA\/RNA Binding: Researchers commonly examine how HMGB3 (High mobility group protein B3) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative HMGB3 (High mobility group protein B3) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of HMGB3 (High mobility group protein B3) across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eIF\/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation\/permeabilization and epitope accessibility.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFamily \/ similarity context:\u003c\/strong\u003e Contains 1 HMG box DNA-binding domain.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for O15347: https:\/\/www.uniprot.org\/uniprotkb\/O15347\/entry - PubMed search (HMGB3): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HMGB3 - NCBI Gene search (HMGB3): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HMGB3 - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066829726061,"sku":"PB9633","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067079516525,"sku":"PB9633-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067079549293,"sku":"PB9633-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067079582061,"sku":"PB9633-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067079614829,"sku":"PB9633-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067079647597,"sku":"PB9633-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067079680365,"sku":"PB9633-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067079713133,"sku":"PB9633-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067079745901,"sku":"PB9633-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067079778669,"sku":"PB9633-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067079811437,"sku":"PB9633-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067079844205,"sku":"PB9633-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pb9633-1-WB-anti-hmg4-picoband-antibody.jpg?v=1772608172","url":"https:\/\/www.ebiohippo.com\/products\/anti-hmg4-hmgb3-antibody-picoband-bha21000534","provider":"BioHippo","version":"1.0","type":"link"}