{"product_id":"anti-human-atf4-creb2-antibody-n360a-24-bha21405806","title":"Anti-Human ATF4\/CREB2 Antibody (N360A\/24)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against ATF4\/CREB2 (Tax-responsive enhancer element-binding protein 67) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eATF4 is a transcription factor or transcriptional regulator that modulates gene-expression programs in response to developmental or environmental cues. These proteins often act downstream of signaling pathways and are assessed for expression level, subcellular localization and pathway activation states.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003ePrimarily nuclear when active; many are regulated by cytoplasmic sequestration and stimulus-dependent nuclear import.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Rat).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of ATF4\/CREB2, and a positive control sample known to express ATF4\/CREB2.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036938985837,"sku":"HB073023-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037551681901,"sku":"HB073023-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037551714669,"sku":"HB073023-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB073023-SDS-PAGE-1.jpg?v=1771746137","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-atf4-creb2-antibody-n360a-24-bha21405806","provider":"BioHippo","version":"1.0","type":"link"}