{"product_id":"anti-human-cd124-il4r-polyclonal-antibody-bha21402010","title":"Anti-Human CD124\/IL4R Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eThis antibody is intended for research detection of \u003cstrong\u003eIL4R\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eIL4R (IL-4-binding protein)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eIL4R (CD124) encodes the interleukin‑4 receptor alpha chain. It forms receptor complexes for IL‑4 (type I, with the common gamma chain) and IL‑4\/IL‑13 (type II, with IL13RA1), driving JAK\/STAT6 signaling and Th2‑associated immune programs. IL4R is widely studied in allergy\/asthma biology, macrophage polarization, and immune regulation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003emembrane-associated (may vary by cell type and experimental conditions).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eCommonly used in \u003cstrong\u003eImmunology \u0026amp; Inflammation\u003c\/strong\u003e workflows to study expression changes, pathway state, and cellular localization of IL4R.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible workflows\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eELISA\u003c\/li\u003e\n\u003cli\u003eIHC\u003c\/li\u003e\n\u003cli\u003eWB\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eWestern blot: optimize sample preparation (reducing vs. non‑reducing and denaturing conditions) and titrate antibody to balance sensitivity and background. Apparent molecular weight may vary with isoforms and post‑translational modifications (e.g., glycosylation or phosphorylation).\u003c\/li\u003e\n\u003cli\u003eIHC: epitope accessibility can depend strongly on fixation and antigen retrieval. Test heat‑induced retrieval buffers (citrate pH ~6 or Tris‑EDTA pH ~9) and adjust retrieval time to improve signal-to-background.\u003c\/li\u003e\n\u003cli\u003eELISA: performance is sensitive to coating conditions and blocking chemistry; optimize capture density and blocking reagent to reduce non‑specific binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eRecommended controls include a positive sample reported to express the target (literature‑validated cell line or tissue), a negative\/low‑expression sample when available, and ideally genetic knockdown\/knockout or peptide‑blocking approaches to confirm specificity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eE. coli - derived recombinant Human CD124\/IL4R (Met26-His232).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003cbr\u003eFor research use only. Not for diagnostic procedures.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036813320557,"sku":"HB732014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037394592109,"sku":"HB732014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB732014-abinscience-WB-1.jpg?v=1771743812","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-cd124-il4r-polyclonal-antibody-bha21402010","provider":"BioHippo","version":"1.0","type":"link"}