{"product_id":"anti-human-cd175-tn-antibody-83d4-bha21405735","title":"Anti-Human CD175\/Tn Antibody (83D4)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against CD175\/Tn (Tn- (GalNAcα1-O-Ser) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eCD175 is a cluster of differentiation (CD) molecule used to phenotype immune cells and characterize activation\/differentiation states. CD markers often function in antigen recognition, costimulation, adhesion or signaling, and are widely used in flow cytometry, immunohistochemistry and immunofluorescence.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eCell-surface (plasma membrane) marker; some CD proteins can be internalized or shed.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eImmunology \u0026amp; Inflammation\u003c\/em\u003e research. Application suitability (e.g., ELISA, IF, SPR, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of CD175\/Tn, and a positive control sample known to express CD175\/Tn.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036937707885,"sku":"HA999043-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037547094381,"sku":"HA999043-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037547127149,"sku":"HA999043-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HA999043-SDSPAGE-1.jpg?v=1771746077","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-cd175-tn-antibody-83d4-bha21405735","provider":"BioHippo","version":"1.0","type":"link"}