{"product_id":"anti-human-cd227-muc1-antibody-hmfg1-fitc-bha21418558","title":"Anti-Human CD227\/MUC1 Antibody (HMFG1), FITC","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nNormal Guinea pig IgG Isotype Control Antibody, PE is designed to match primary antibody format for background estimation. Commonly used in FCM, IF, Isotype Control applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Detect target localization by IF\/ICC in cultured cells, Phenotype target-positive cells by flow cytometry in PBMCs\/cell suspensions, Build multiplex staining panel using conjugated antibody and controls, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Guinea pig; Clonality: Polyclonal; Isotype: IgG; Conjugate: PE; Format: Liquid.\u003c\/p\u003e\n\n\u003ch3\u003eIsotype control rationale\u003c\/h3\u003e\n\u003cp\u003eIsotype control antibodies are matched to the primary antibody’s species and isotype, but lack specificity for a cellular antigen. They are used to estimate non‑specific binding and background signal in immunoassays. In flow cytometry and immunofluorescence, isotype controls can help identify staining driven by Fc receptor interactions, sticky epitopes, or fluorophore\/label-associated background rather than true antigen recognition. When conjugated, the isotype control should be used with the same fluorophore\/label and at the same concentration as the corresponding test antibody. This reagent is characterized by its host species (Guinea pig), isotype (IgG), which should match your primary antibody format as closely as possible.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eUse this isotype control to estimate non-specific background under matched staining conditions. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eRun the isotype control at the same concentration, incubation time, and staining conditions as the test antibody.\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, consider Fc blocking and use an FMO (fluorescence minus one) control to support gating decisions.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, include Fc blocking when applicable, use a viability dye, and consider staining live cells for surface markers prior to fixation\/permeabilization.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eStore at 4°C for 12 months. Protect from light. Do not freeze. 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 T","offer_id":53037271581037,"sku":"HB102517-100T","price":370.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-human-cd227-muc1-antibody-hmfg1-fitc-bha21418558","provider":"BioHippo","version":"1.0","type":"link"}