{"product_id":"anti-human-cd257-baff-bha14700104","title":"anti-human CD257(BAFF)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n  \u003cspan class=\"bhp-cat-pill\"\u003ePrimary Antibody — Monoclonal\u003c\/span\u003e\n\n  \u003ch2\u003eProduct Overview\u003c\/h2\u003e\n  \u003cp\u003eAntibody ANC2H3 binds to CD257(BAFF) in EIA and  blocks binding of recombinant CD257(BAFF)-muCD8 to receptors on Raji cell surface in Flow cytometry.\u003c\/p\u003e\n\n  \u003cp\u003eHuman CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2).  BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells(3,4).  Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1).  A third receptor CD268 (BAFFR) is specific for BAFF has also been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFFR may the primary receptor for BAFF in mice(8,9,10).  Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren’s Syndrome(11).  Clone ANC2H3 binds to recombinant BAFF in EIA and Flow Cytometry and blocks binding of recombinant human CD257(BAFF)trn-muCD8 (catalog #525-020)  to receptors on Raji cells in Flow Cytometry.\u003c\/p\u003e\n\n  \u003ch2\u003eValidated Applications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eEIA\u003c\/td\u003e\n\u003ctd\u003eSee datasheet\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/table\u003e\n\n  \u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eClone\u003c\/th\u003e\n\u003ctd\u003eANC2H3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG1 kappaappa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eBiotin, FITC, PE, Unconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 2 - 5°C. Freeze\/Thawing is not recommended.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/table\u003e\n\n  \u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n  \u003cp\u003eRecombinant soluble human CD257(BAFF)\u003c\/p\u003e\n\n  \u003ch2\u003eFunctional Activity\u003c\/h2\u003e\n  \u003cp\u003eAntibody ANC2H3 blocks binding of Recombinant CD257(BAFF) to Raji cell surface.\u003c\/p\u003e\n\n  \u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n  \u003cdiv class=\"bhp-warning-box\"\u003e\n    \u003cp\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use. \n    Handle as a potential biohazard in accordance with good laboratory practices.\u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003ch2\u003eRelated Products\u003c\/h2\u003e\n  \u003cp\u003eExplore complementary antibodies, ELISA kits, and detection reagents at \n  \u003ca href=\"https:\/\/www.ebiohippo.com\" target=\"_blank\"\u003eBioHippo\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Ancell Corporation","offers":[{"title":"Purified (with Antibiotic) \/ 100 ug","offer_id":53328072147309,"sku":"266-020","price":282.0,"currency_code":"USD","in_stock":true},{"title":"Biotin conjugate \/ 100 ug","offer_id":53328104522093,"sku":"266-030","price":390.0,"currency_code":"USD","in_stock":true},{"title":"FITC (Fluorescein) conjugate \/ 120 tests","offer_id":53328104554861,"sku":"266-040","price":390.0,"currency_code":"USD","in_stock":true},{"title":"PE (Phycoerythrin) conjugate \/ 120 tests","offer_id":53328104587629,"sku":"266-050","price":420.0,"currency_code":"USD","in_stock":true},{"title":"Purified (Preservative-free) \/ 100 ug","offer_id":53328104620397,"sku":"266-820","price":282.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-human-cd257-baff-bha14700104","provider":"BioHippo","version":"1.0","type":"link"}