{"product_id":"anti-human-cd274-pd-l1-b7-h1-antibody-sp263-bha21405698","title":"Anti-Human CD274\/PD-L1\/B7-H1 Antibody (SP263)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against CD274\/PD-L1\/B7-H1 (Programmed cell death 1 ligand 1) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eCD274 is a cluster of differentiation (CD) molecule used to phenotype immune cells and characterize activation\/differentiation states. CD markers often function in antigen recognition, costimulation, adhesion or signaling, and are widely used in flow cytometry, immunohistochemistry and immunofluorescence.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eCell-surface (plasma membrane) marker; some CD proteins can be internalized or shed.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eImmunology \u0026amp; Inflammation\u003c\/em\u003e research. Application suitability (e.g., IHC) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of CD274\/PD-L1\/B7-H1, and a positive control sample known to express CD274\/PD-L1\/B7-H1.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036936495469,"sku":"HV974083-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037544210797,"sku":"HV974083-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037544243565,"sku":"HV974083-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HV974083-SDSPAGE-1.jpg?v=1771746080","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-cd274-pd-l1-b7-h1-antibody-sp263-bha21405698","provider":"BioHippo","version":"1.0","type":"link"}