{"product_id":"anti-human-chrna1-achra-antibody-mab35-apc-bha21411307","title":"Anti-Human CHRNA1, ACHRA Antibody (Mab35), APC","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Human CHRNA1, ACHRA Antibody (Mab35), APC is intended for detection of \u003cstrong\u003eHuman CHRNA1, ACHRA\u003c\/strong\u003e. Commonly used in FCM applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Phenotype target-positive cells by flow cytometry in PBMCs\/cell suspensions, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Acetylcholine receptor subunit alpha, CHRNA1, ACHRA, CHNRA.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Mouse; Clonality: Monoclonal; Isotype: IgG1, kappa; Conjugate: APC; Format: Liquid; Reactivity: Human.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eHuman CHRNA1, ACHRA (Acetylcholine receptor subunit alpha) is a modification-specific epitope used to assess protein regulatory state rather than total protein abundance. Post-translational modification epitopes can be stimulation- and fixation-sensitive; rapid sample processing and appropriate inhibitors can help preserve signal. Common alternative names and symbols used in the literature include: CHRNA1, ACHRA, CHNRA. Based on the annotated name\/class, the antigen is most often associated with plasma membrane and\/or intracellular membranes localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, include Fc blocking when applicable, use a viability dye, and consider staining live cells for surface markers prior to fixation\/permeabilization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eStore at 4°C for 12 months. Protect from light. Do not freeze. 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 T","offer_id":53037122224493,"sku":"HF829137-100T","price":482.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-human-chrna1-achra-antibody-mab35-apc-bha21411307","provider":"BioHippo","version":"1.0","type":"link"}