{"product_id":"anti-human-fn1-fibronectin-antibody-saa0522-bha21408518","title":"Anti-Human FN1\/Fibronectin Antibody (SAA0522)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Human FN1\/Fibronectin Antibody (SAA0522) targets \u003cstrong\u003eFN1\/Fibronectin\u003c\/strong\u003e (Cold-insoluble globulin). Frequently used across ELISA, IF, IHC, SPR, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in ECM \u0026amp; Cell Adhesion research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Cold-insoluble globulin, Fibronectin, CIG, FN1, FN.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eFN1\/Fibronectin (Cold-insoluble globulin) is annotated as cold-insoluble globulin and is commonly profiled for expression and localization in relevant model systems. It is frequently investigated in contexts related to ECM \u0026amp; Cell Adhesion. Common alternative names and symbols used in the literature include: Fibronectin, CIG, FN1, FN. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037031620973,"sku":"HY259013-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037734658413,"sku":"HY259013-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037734691181,"sku":"HY259013-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HY259013-SDS-PAGE-1.jpg?v=1771747859","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-fn1-fibronectin-antibody-saa0522-bha21408518","provider":"BioHippo","version":"1.0","type":"link"}