{"product_id":"anti-human-glycosylated-cd133-prom1-antibody-ac133-1-bha21405526","title":"Anti-Human Glycosylated CD133\/PROM1 Antibody (AC133\/1)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against Glycosylated CD133\/PROM1 (Prominin-like protein 1) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eGLYCOSYLATED is a molecular target commonly interrogated by antibody-based methods to measure protein expression, localization and regulation across biological systems. Antibodies against GLYCOSYLATED support studies ranging from baseline expression profiling to stimulus- or disease-associated changes in abundance and subcellular distribution. For pathway-level interpretation, pair GLYCOSYLATED detection with appropriate controls and complementary readouts (e.g., genetic perturbation or orthogonal assays).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eSubcellular localization is target- and context-dependent; use cell-type appropriate controls and, where possible, genetic KO\/KD as specificity controls.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eImmunology \u0026amp; Inflammation\u003c\/em\u003e research. Application suitability (e.g., FCM, ICC\/IF, IF, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of Glycosylated CD133\/PROM1, and a positive control sample known to express Glycosylated CD133\/PROM1.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036929909101,"sku":"HT495023-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037533299053,"sku":"HT495023-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037533331821,"sku":"HT495023-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-human-glycosylated-cd133-prom1-antibody-ac133-1-bha21405526","provider":"BioHippo","version":"1.0","type":"link"}