{"product_id":"anti-human-map3k2-antibody-e6-bha21405816","title":"Anti-Human MAP3K2 Antibody (E6#)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against MAP3K2 (Mitogen-activated protein kinase kinase kinase 2) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eMAP3K2 is part of the MAP kinase signaling architecture that transduces extracellular cues into phosphorylation cascades controlling gene expression, proliferation, differentiation and stress responses. MAPK pathway components are frequently interrogated to understand stimulus-dependent signaling dynamics and drug response in oncology and inflammatory models.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eMAPK pathway proteins often shuttle between cytoplasm and nucleus upon activation and can be enriched at signaling complexes.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eCell Signaling\u003c\/em\u003e research. Application suitability (e.g., ELISA, SPR, WB) is indicated in the specifications; conditions should be optimized by titration.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of MAP3K2, and a positive control sample known to express MAP3K2.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036939772269,"sku":"HA034013-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037552599405,"sku":"HA034013-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037552632173,"sku":"HA034013-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HA034013-SDS-PAGE-1.jpg?v=1771746144","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-map3k2-antibody-e6-bha21405816","provider":"BioHippo","version":"1.0","type":"link"}