{"product_id":"anti-human-mda5-ifih1-antibody-saa2313-bha21404987","title":"Anti-Human MDA5\/IFIH1 Antibody (SAA2313)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against MDA5\/IFIH1 (Interferon-induced helicase C domain-containing protein 1) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eInterferon-induced helicase C domain-containing protein 1 is a helicase-family ATPase that remodels nucleic acids and\/or ribonucleoprotein complexes. Helicases are central to replication, transcription, splicing and DNA damage responses and are frequently studied in genome maintenance and RNA biology.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eOften nuclear (DNA\/RNA processing sites), with shuttling or cytoplasmic pools depending on function.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eOxidative Stress\u003c\/em\u003e research. Application suitability (e.g., ELISA, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of MDA5\/IFIH1, and a positive control sample known to express MDA5\/IFIH1.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036912705901,"sku":"HV365033-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037497876845,"sku":"HV365033-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037497909613,"sku":"HV365033-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HV365033-WB-1.jpg?v=1771745667","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-mda5-ifih1-antibody-saa2313-bha21404987","provider":"BioHippo","version":"1.0","type":"link"}