{"product_id":"anti-human-ppm1d-polyclonal-antibody-bha21400385","title":"Anti-Human PPM1D Polyclonal Antibody","description":"\u003ch3\u003eOverview\u003c\/h3\u003e\u003cp\u003eThis antibody enables detection of \u003cstrong\u003ePPM1D\u003c\/strong\u003e (Protein phosphatase magnesium-dependent 1 delta) \u003cspan style=\"color:#555\"\u003e(also known as Protein phosphatase 1D, 3.1.3.16, Protein phosphatase 2C isoform delta, PP2C-delta)\u003c\/span\u003e in Human samples. It is designed to support ELISA, IHC, WB and related assays where specific target recognition is required.\u003c\/p\u003e\u003ch3\u003eTarget biology\u003c\/h3\u003e\u003cp\u003eProtein phosphatase magnesium-dependent 1 delta is a phosphatase that counterbalances kinase activity by removing phosphate groups from proteins or metabolites. Phosphatases help tune signaling amplitude and duration in pathways controlling growth and immune activation. Based on protein class and annotation keywords, it is most often associated with the \u003cstrong\u003evaries by cell type and isoform\u003c\/strong\u003e. This target is commonly used in \u003cstrong\u003eCell Signaling\u003c\/strong\u003e research.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization:\u003c\/strong\u003e varies by cell type and isoform (may vary with cell type, activation state, and isoform).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context:\u003c\/strong\u003e Frequently used in Cell Signaling studies.\u003c\/p\u003e\u003ch3\u003eImmunogen\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eImmunogen context:\u003c\/strong\u003e Raised against E. coli - derived recombinant Human PPM1D (Ser96-Leu395).. Epitope accessibility can vary by assay format and sample treatment, so titration is recommended.\u003c\/p\u003e\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTitrate for your system:\u003c\/strong\u003e Start with a mid-range working concentration and optimize for signal-to-background in your exact sample type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUse biological controls:\u003c\/strong\u003e Where possible, include a known positive sample and a target-low\/negative sample (or KO\/KD) to confirm specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInterpret bands\/staining carefully:\u003c\/strong\u003e Many targets have isoforms, PTMs, or processing that can change apparent size or localization; corroborate with orthogonal readouts when available.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Optimize lysis\/denaturation conditions for epitope accessibility; include a positive lysate and consider reducing vs non-reducing conditions if the target is disulfide-rich or multimeric.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Fixation and antigen retrieval can strongly influence staining. Start with a standard heat-induced retrieval condition and titrate antibody to minimize background; include known positive and negative tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e For best sensitivity, optimize capture\/detection pairing and blocking conditions; test recombinant standard curves and matrix effects for your sample type.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036758401389,"sku":"HT072014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037337739629,"sku":"HT072014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HT072014-abinscience-WB-1.jpg?v=1771742897","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-ppm1d-polyclonal-antibody-bha21400385","provider":"BioHippo","version":"1.0","type":"link"}