{"product_id":"anti-human-tp53-p53-r175h-mutant-antibody-f2-pe-bha21419403","title":"Anti-Human TP53\/p53 (R175H mutant) Antibody (F2), PE","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Human TP53\/p53 (R175H mutant) Antibody (F2), PE is intended for detection of \u003cstrong\u003eHuman TP53\/p53\u003c\/strong\u003e. Commonly used in ELISA, FCM applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Phenotype target-positive cells by flow cytometry in PBMCs\/cell suspensions, Develop ELISA pairing using capture\/detection antibodies and a standard curve, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Oncology \u0026amp; Angiogenesis.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Cellular tumor antigen p53, Phosphoprotein p53, Antigen NY-CO-13, Tumor suppressor p53, TP53, P53.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a, kappa; Conjugate: PE; Format: Liquid; Reactivity: Human.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eHuman TP53\/p53 (Cellular tumor antigen p53) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Oncology \u0026amp; Angiogenesis. Common alternative names and symbols used in the literature include: Phosphoprotein p53, Antigen NY-CO-13, Tumor suppressor p53, TP53, P53. Based on the annotated name\/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, include Fc blocking when applicable, use a viability dye, and consider staining live cells for surface markers prior to fixation\/permeabilization.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eStore at 4°C for 12 months. Protect from light. Do not freeze. 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 T","offer_id":53037299761517,"sku":"HY039127-100T","price":424.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-human-tp53-p53-r175h-mutant-antibody-f2-pe-bha21419403","provider":"BioHippo","version":"1.0","type":"link"}