{"product_id":"anti-human-trim28-nanobody-saa1235-bha21407953","title":"Anti-Human TRIM28 Nanobody (SAA1235)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Human TRIM28 Nanobody (SAA1235) targets \u003cstrong\u003eTRIM28\u003c\/strong\u003e (RING-type E3 ubiquitin transferase TIF1-beta). Frequently used across ELISA, IP, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Protein Homeostasis \u0026amp; Degradation research.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eTRIM28 (RING-type E3 ubiquitin transferase TIF1-beta) encodes an E3 ubiquitin ligase component that tags substrates with ubiquitin to regulate stability, trafficking, or signaling. Ubiquitin pathway proteins are commonly evaluated in proteostasis and signal turnover mechanisms. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037011960173,"sku":"HC420023-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037697761645,"sku":"HC420023-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037697794413,"sku":"HC420023-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HC420023-SDS-PAGE-1.jpg?v=1771747593","url":"https:\/\/www.ebiohippo.com\/products\/anti-human-trim28-nanobody-saa1235-bha21407953","provider":"BioHippo","version":"1.0","type":"link"}