{"product_id":"anti-kcnb1-drk1-antibody-internal-stj71320-bha15528954","title":"Anti-KCNB1\/DRK1 antibody (Internal) (STJ71320)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePolyclonal Antibody\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis polyclonal antibody, raised in Goat, recognizes KCNB1. It is validated for Pep-ELISA.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003ePep-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePeptide ELISA\u003c\/th\u003e\n\u003ctd\u003eantibody detection limit dilution 1:4000.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eWB\u003c\/th\u003e\n\u003ctd\u003ePreliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5µg\/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field-have any results been reported with other antibodies\/lysates? Have any further splice variants\/modified forms been reported?\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eOptimal dilutions should be determined by the end user for each application and sample type.\u003c\/p\u003e\n\u003ch2\u003eImmunogen\u003c\/h2\u003e\n\u003cp\u003eSee specifications table.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Dog, Cow, Pig\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen Region\u003c\/th\u003e\n\u003ctd\u003eInternal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003ePurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e0.5 mg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003e0.5 mg\/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene ID\u003c\/th\u003e\n\u003ctd\u003e3745\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at-20°C on receipt and minimise freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eValidated reactivity: Human, Mouse, Dog, Cow, Pig. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eThis product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore related antibodies and reagents for KCNB1 research from St John's Laboratory Ltd, or contact us for alternative formats.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"100 ug","offer_id":53718884286829,"sku":"STJ71320-100","price":276.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-kcnb1-drk1-antibody-internal-stj71320-bha15528954","provider":"BioHippo","version":"1.0","type":"link"}