{"product_id":"anti-keap1-antibody-picoband-bha21003795","title":"Anti-KEAP1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-KEAP1 Antibody Picoband® is an antibody for KEAP1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e KEAP1 (cyclin-dependent kinase 5); UniProt: Q14145\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 66-72 kDa, calculated 40108 MW\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, IHC, IF, ICC, Flow Cytometry, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-KEAP1 Antibody Picoband® catalog # A00514-3.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e Proline-ed serine\/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM\/vimentin, p35\/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM\/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP\/NFAT5, MAPT\/TAU, MAP1B, histone H1, p53\/TP53, HDAC1, APEX1, PTK2\/FAK1, huntingtin\/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT\/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and\/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM\/MUC18, SEPT5, SYN1, and CDK16\/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3\/MAPK10 activity. Phosphorylation of p53\/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53\/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35\/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25\/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25\/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin\/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2\/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt\/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP\/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35\/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL\/BMAL1 heterodimer in association with altered stability and subcellular distribution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e cellular localization: Cell membrane. Peripheral membrane protein. Postsynaptic density. Cytoplasm. Perikaryon. Lamellipodium. Growth cone., tissue context: Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare KEAP1 levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Evaluate spatial distribution of KEAP1 in tissue sections, considering fixation and antigen retrieval effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence \/ ICC:\u003c\/strong\u003e Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify KEAP1-positive populations in single-cell suspensions with appropriate gating and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB\/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Cell membrane. Peripheral membrane protein. Postsynaptic density. Cytoplasm. Perikaryon. Lamellipodium. Growth cone.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e Cofactors, Vitamins\/Minerals,Hematopoietic Progenitors,Metabolism,Myeloid,Pathways and Processes,Signal Transduction,Stem Cells,Vitamins\/Minerals\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for Q14145: https:\/\/www.uniprot.org\/uniprot\/Q14145 - Ensembl search (KEAP1): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=KEAP1 - Human Protein Atlas search (KEAP1): https:\/\/www.proteinatlas.org\/search\/KEAP1 - PubMed search (KEAP1): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=KEAP1 --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067759812973,"sku":"A00514-3","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53068410487149,"sku":"A00514-3-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53068410519917,"sku":"A00514-3-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53068410552685,"sku":"A00514-3-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53068410585453,"sku":"A00514-3-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53068410618221,"sku":"A00514-3-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53068410650989,"sku":"A00514-3-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53068410683757,"sku":"A00514-3-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53068410716525,"sku":"A00514-3-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53068410749293,"sku":"A00514-3-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53068410782061,"sku":"A00514-3-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53068410814829,"sku":"A00514-3-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a00514-3-keap1-primary-antibodies-wb-testing-1_1.jpg?v=1772609885","url":"https:\/\/www.ebiohippo.com\/products\/anti-keap1-antibody-picoband-bha21003795","provider":"BioHippo","version":"1.0","type":"link"}