{"product_id":"anti-kir4-1-kcnj10-extracellular-antibody-bha21301058","title":"Anti-Kir4.1 (KCNJ10) (extracellular) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-Kir4.1 (KCNJ10) (extracellular) Antibody is an antibody targeting ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10 (also reported as ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Extracellular loop.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Mouse - identical; human - 13\/14 amino acid residues identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecificity statement (as provided):\u003c\/strong\u003e May have some cross-reactivity with rat Kir4.2 (Accession Q91ZF1)..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eCited use:\u003c\/strong\u003e IHC (literature use does not guarantee performance in every setup).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eKir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eLinking transporter\/channel abundance to ionic homeostasis and excitability-related phenotypes.\u003c\/li\u003e   \u003cli\u003eStudying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.\u003c\/li\u003e   \u003cli\u003eCombining antibody readouts with functional assays for more complete interpretation.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e   \u003cli\u003eImmunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence\/ICC: assess subcellular localization and co-localization with markers in cells or sections.\u003c\/li\u003e   \u003cli\u003eLive cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-PC165.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-PC165; Negative control: BLP-PC165.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064850112877,"sku":"APC-165-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064960442733,"sku":"APC-165-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064960475501,"sku":"APC-165-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064960508269,"sku":"APC-165-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/APC-165_ept.gif?v=1772461061","url":"https:\/\/www.ebiohippo.com\/products\/anti-kir4-1-kcnj10-extracellular-antibody-bha21301058","provider":"BioHippo","version":"1.0","type":"link"}