{"product_id":"anti-lrrc26-antibody-bha21300993","title":"Anti-LRRC26 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-LRRC26 Antibody is an antibody targeting Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC (also reported as Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Intracellular, C-terminus.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Mouse - 12\/14 amino acid residues identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecificity statement (as provided):\u003c\/strong\u003e Won’t recognize human LRRC26..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eBK (KCa1.1) channels are critically involved in diverse physiological processes in both electrically excitable and non-excitable cells.Leucine-rich repeat containing protein 26 (also known as BK channel auxiliary gamma subunit or LRRC26) is a single spanning transmembrane protein of ~37 kDa. LRRC26 protein contains five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex.When LRRC26 is acutely knocked down in LNCaP cells, the voltage-dependent activation of endogenous BK current shifts from a low to a high V1\/2 value, whereas recombinant expression of LRRC26 cDNA in naïve cells (such as PC3 prostate cancer cells and HEK 293 cells) normally lacking LRRC26 shifts BK channel activation from high to low V1\/2 values, reproducing the channel behavior observed in LNCaP cells.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eLinking transporter\/channel abundance to ionic homeostasis and excitability-related phenotypes.\u003c\/li\u003e   \u003cli\u003eStudying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.\u003c\/li\u003e   \u003cli\u003eCombining antibody readouts with functional assays for more complete interpretation.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-PC070.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-PC070; Negative control: BLP-PC070.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064847491437,"sku":"APC-070-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064954478957,"sku":"APC-070-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064954511725,"sku":"APC-070-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064954544493,"sku":"APC-070-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/APC-070_ept-1.gif?v=1772461055","url":"https:\/\/www.ebiohippo.com\/products\/anti-lrrc26-antibody-bha21300993","provider":"BioHippo","version":"1.0","type":"link"}