{"product_id":"anti-macrosialin-cd68-antibody-picoband-bha21006858","title":"Anti-Macrosialin CD68 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-Macrosialin CD68 Antibody Picoband® is an antibody targeting \u003cstrong\u003eCD68\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, IF, Flow Cytometry, ELISA\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eMouse,Rat\u003c\/strong\u003e; observed MW: \u003cstrong\u003e90-100 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e34818 MW\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp class=\"text-grey\" mb-2\u003e\u003cstrong\u003eSuperstar antibody:\u003c\/strong\u003e Boster’s anti mouse CD68 antibody (PA1518) is among its top 10 bestselling antibodies, and one of the most cited mouse\/rat CD68 antibodies on the market. Because of this antibody’s high demand, we have subsequently developed \u003ca href=\"\/anti-cd68-rabbit-monoclonal-antibody-m00602-1-boster.html\" target=\"_blank\"\u003ea rabbit monoclonal anti CD68 antibody (M00602-1)\u003c\/a\u003e targetting a similar epitope.\u003c\/p\u003e \u003cp class=\"text-grey\" mb-2\u003e\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e PA1518 reacts with murine macrosialin\/CD68, and has been validated with Western blotting and confirmed its stellar specificity. CD68 is a highly glycosylated protein, and its expected western blot molecular weight is between 80kDa to 110kDa, depending on glycosylation level. PA1518’s observed MW in WB is near 90-100kDa. Testing on negative control tissues showed no significant bands (images available on request) or staining. See more info in the positive and negative control design section. Based on immunogen sequence homology, this antibody is not expected to cross react with other proteins from the LAMP family, which complies with QC testing observations. \u003c\/p\u003e \u003cp class=\"text-grey\" mb-2\u003e\u003cstrong\u003eGreat for CD68 IHC:\u003c\/strong\u003e This antibody produces clean and specific immunobiological stains in both mouse and rat tissues. Our QC team has validated it on spleen and liver tissues of both mice and rats. Click product images for more details on experiment conditions.\u003c\/p\u003e \u003cp class=\"text-grey\" mb-2\u003e\u003cstrong\u003eMore about CD68:\u003c\/strong\u003e CD68 is a cell surface marker often used to identify macrophages and other cell types in the monocyte lineage. It is a transmembrane protein that binds to electins and selectins and plays a role in macrophage homing movement. Check out the \u003ca href=\"\/bosterbio-gene-info-cards\/CD68\" target=\"_blank\"\u003eCD68 \u003c\/a\u003ebiomarker page for more information on CD68 and view all CD68 antibodies, ELISA kits and proteins. \u003c\/p\u003e The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD68 — Macrosialin\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Mouse,Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 90-100 kDa; Calculated: 34818 MW\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be ed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details (datasheet):\u003c\/strong\u003e Expressed in tissue macrophages and to a lesser extent in dendritic cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Belongs to the LAMP family.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Cell Type Markers,Immunology.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence \/ ICC:\u003c\/strong\u003e Visualize subcellular localization and co-localization patterns; consider fixation\/permeabilization compatibility and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069791330669,"sku":"PA1518","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070145683821,"sku":"PA1518-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070145716589,"sku":"PA1518-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070145749357,"sku":"PA1518-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070145782125,"sku":"PA1518-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070145814893,"sku":"PA1518-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070145847661,"sku":"PA1518-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070145880429,"sku":"PA1518-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070145913197,"sku":"PA1518-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070145945965,"sku":"PA1518-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070145978733,"sku":"PA1518-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070146011501,"sku":"PA1518-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pa1518-cd68-primary-antibodies-wb-testing-1_1.jpg?v=1772612557","url":"https:\/\/www.ebiohippo.com\/products\/anti-macrosialin-cd68-antibody-picoband-bha21006858","provider":"BioHippo","version":"1.0","type":"link"}