{"product_id":"anti-magi2-aip1-antibody-bha21301190","title":"Anti-MAGI2\/AIP1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-MAGI2\/AIP1 Antibody is an antibody targeting Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1 (also reported as Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Intracellular, C-terminus.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Rat, human - identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Unconjugated (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eMAGI2 is a member in the Membrane-associated guanylate kinase with an inverted arrangement of protein-protein interaction domains (MAGI) family, and includes two additional family members, MAGI1 and MAGI31.MAGI proteins are essential for efficient cell signaling as they function as scaffolds for assembling cell adhesion molecules, receptors and signaling molecules and linking them to the cytoskeleton2.MAGI2 has a guanylate kinase, 2 WW, and 6 PDZ domains (PDZ0-PDZ5). It interacts with cytoskeleton-associated proteins: guanylate-kinase-associated protein (GKAP), Postsynaptic density-95 (PSD-95)\/synapse-associated protein 90 (SAP90), Stargazin\/transmembrane AMPAR regulatory proteins (TARPs). MAGI2 also interacts with N-methyl-D-aspartate (NMDA) receptors, δ2 Glutamate receptor, β1AR, VPAC1, Brain-specific angiogenesis inhibitor-1, Activin Type II receptors, Neuroligins, β-Dystroglycan, Dendrite arborization and synapse maturation 1 (Dasm1)\/IgSF9b, Nectins and Nephrin receptors and cell adhesion proteins, respectively1,3.MAGI2 expression is almost exclusively in brain, deletion of MAGI2 causes infantile spasm and increased cognitive impairment in schizophrenia patients4.In non-neuronal tissues, MAGI2 enhances the ability of PTEN to suppress Akt activation in the PI3-kinase\/Akt pathway.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eComparing target expression across perturbations, genotypes, or treatment conditions.\u003c\/li\u003e   \u003cli\u003eInterpreting localization shifts alongside pathway or phenotypic readouts.\u003c\/li\u003e   \u003cli\u003eUsing orthogonal controls (KO\/KD, peptide competition, isotype concepts) to support conclusions.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-PZ052.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-PZ052; Negative control: BLP-PZ052.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064853815661,"sku":"APZ-052-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064972632429,"sku":"APZ-052-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064972665197,"sku":"APZ-052-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064972697965,"sku":"APZ-052-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/APZ-052_ept.gif?v=1772461077","url":"https:\/\/www.ebiohippo.com\/products\/anti-magi2-aip1-antibody-bha21301190","provider":"BioHippo","version":"1.0","type":"link"}