{"product_id":"anti-matrix-metalloproteinase-16-mmp16-antibody-picoband-bha21006669","title":"Anti-Matrix metalloproteinase-16 MMP16 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-Matrix metalloproteinase-16 MMP16 Antibody Picoband® is an antibody targeting \u003cstrong\u003eMMP16\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, Flow Cytometry, ELISA, IHC-F\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eHuman,Rat\u003c\/strong\u003e; observed MW: \u003cstrong\u003e70 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e69521 MW\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-Matrix metalloproteinase-16 MMP16 Antibody catalog # PA1123. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MMP16 — Matrix metalloproteinase-16\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human,Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 70 kDa; Calculated: 69521 MW\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Isoform Long: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details (datasheet):\u003c\/strong\u003e Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Belongs to the peptidase M10A family.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Invasion\/Microenvironment,Metalloprotease,Proteolysis\/Ubiquitin,Proteolytic Enzymes.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069785891181,"sku":"PA1123","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070075330925,"sku":"PA1123-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070075363693,"sku":"PA1123-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070075396461,"sku":"PA1123-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070075429229,"sku":"PA1123-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070075461997,"sku":"PA1123-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070075494765,"sku":"PA1123-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070075527533,"sku":"PA1123-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070075560301,"sku":"PA1123-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070075593069,"sku":"PA1123-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070075625837,"sku":"PA1123-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070075658605,"sku":"PA1123-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pa1123-mmp16-primary-antibodies-wb-testing-1_1.jpg?v=1772612548","url":"https:\/\/www.ebiohippo.com\/products\/anti-matrix-metalloproteinase-16-mmp16-antibody-picoband-bha21006669","provider":"BioHippo","version":"1.0","type":"link"}