{"product_id":"anti-med21-polyclonal-antibody-bha21416642","title":"Anti-MED21 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-MED21 Polyclonal Antibody targets \u003cstrong\u003eMED21\u003c\/strong\u003e (Mediator of RNA polymerase II transcription subunit 21). Frequently used across ELISA, IHC, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Molecular \u0026amp; Cellular Biology research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Mediator of RNA polymerase II transcription subunit 21, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, Mediator complex subunit 21, hSrb7, SURB7, SRB7.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eMED21 (Mediator of RNA polymerase II transcription subunit 21) is annotated as mediator of rna polymerase ii transcription subunit 21 and is commonly profiled for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular \u0026amp; Cellular Biology. Common alternative names and symbols used in the literature include: RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, Mediator complex subunit 21, hSrb7, SURB7, SRB7. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a polyclonal reagent, this antibody can provide strong sensitivity through recognition of multiple epitopes on the target. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eAntigen \/ immunogen context\u003c\/h3\u003e\u003cp\u003eE. coli - derived recombinant human MED21 (Met1-Ser144).\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037210239341,"sku":"HB423014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037848232301,"sku":"HB423014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB423014-abinscience-WB-1.jpg?v=1771749636","url":"https:\/\/www.ebiohippo.com\/products\/anti-med21-polyclonal-antibody-bha21416642","provider":"BioHippo","version":"1.0","type":"link"}