{"product_id":"anti-mesothelin-car-lentivirus-p4-scfv-cd8-4-1bb-cd3-zeta-bhv18200010","title":"Anti-Mesothelin CAR Lentivirus (P4 ScFv-CD8-4-1BB-CD3ζ)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eMesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eHIV-based · VSV-G-pseudotyped · SIN=Yes\u003c\/span\u003e\u003cp\u003eThe Anti-mesothelin CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain fragment variable) of anti-mesothelin (Clone P4) linked to a 2\u003csup\u003end\u003c\/sup\u003e generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains. A \u003cimg src=\"{{media%20url=\" wysiwyg alt=\"\" width=\"372\" height=\"297\"\u003e B \u003cimg src=\"{{media%20url=\" wysiwyg alt=\"\" width=\"390\" height=\"100\"\u003e Figure 1. (A) Schematic of the lenti-vector used to generate the anti-mesothelin CAR lentivirus and (B) Construct diagram showing components of the anti-mesothelin CAR.\u003c\/p\u003e\n\u003ch2\u003eTechnical Details\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eVector Type\u003c\/th\u003e\n\u003ctd\u003eHIV-based, VSV-G-pseudotyped lentiviral vector\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePayload \/ Construct\u003c\/th\u003e\n\u003ctd\u003eP4 ScFv-CD8-4-1BB-CD3ζ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Antigen \/ Gene\u003c\/th\u003e\n\u003ctd\u003eMesothelin (MSLN \/ MPF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSignaling \/ Architecture\u003c\/th\u003e\n\u003ctd\u003eCD8 hinge \u0026amp; TM · 4-1BB · CD3ζ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSelection Marker\u003c\/th\u003e\n\u003ctd\u003ePuromycin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter\u003c\/th\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiosafety Level\u003c\/th\u003e\n\u003ctd\u003eBSL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSIN Vector\u003c\/th\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eThe lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied As\u003c\/th\u003e\n\u003ctd\u003e50 µl of anti-mesothelin CAR at a titer ≥ 3x10\u003csup\u003e8\u003c\/sup\u003e TU\/ml. The titer will vary with each lot; the exact value is provided with each shipment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e−80°C; avoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHazardous Shipping\u003c\/th\u003e\n\u003ctd\u003eUN3373\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003ePositive control for anti-mesothelin CAR evaluation in T cells; useful for transduction optimization.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eBiosafety \u0026amp; Safety\u003c\/h2\u003e\n\u003cp\u003eThe lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.\u003c\/p\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003e⚠ Safety Note:\u003c\/strong\u003e Avoid freeze\/thaw cycles.\u003c\/div\u003e\n\u003ch2\u003eLicense \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eLicense Disclosure: Visitbpsbioscience.com\/license for the label license and other key information about this product. Troubleshooting Guide: Visit bpsbioscience.com\/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"50 uL","offer_id":53322952933741,"sku":"78703","price":1210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/78703_2.jpg?v=1778643137","url":"https:\/\/www.ebiohippo.com\/products\/anti-mesothelin-car-lentivirus-p4-scfv-cd8-4-1bb-cd3-zeta-bhv18200010","provider":"BioHippo","version":"1.0","type":"link"}