{"product_id":"anti-mlph-antibody-picoband-bha21006154","title":"Anti-MLPH Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-MLPH Antibody Picoband® is an antibody reagent for detection of \u003cstrong\u003eMLPH\u003c\/strong\u003e. Researchers commonly use anti-MLPH antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, IF, ICC, Flow, ELISA).\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-MLPH Antibody Picoband® catalog # A06081-3. Tested in WB, ICC\/IF, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MLPH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Polyclonal; IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies context:\u003c\/strong\u003e Host: Rabbit, Reactivity: Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Immunogen affinity purified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e E.coli-derived human Melanophilin\/MLPH recombinant protein (Position: E61-L523). Human Melanophilin\/MLPH shares 61.6% amino acid (aa) sequence identity with mouse Melanophilin\/MLPH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e observed 85 kDa (reported)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProvided application(s):\u003c\/strong\u003e WB, IHC, IF, ICC, Flow, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThese attributes help contextualize how the antibody is commonly selected (host\/clonality\/isotype\/label) and how signals are interpreted across sample types and assay formats.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eMLPH is commonly studied as part of broader cellular pathways and regulatory networks. Expression level, localization, and isoform context can vary by cell type, state, and stimulus, so interpretation typically considers biological context alongside assay controls.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Melanophilin is a carrier protein which in humans is encoded by the MLPH gene. This gene encodes a member of the exophilin subfamily of Rab effector proteins. The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. A similar protein complex in mouse functions to tether pigment-producing organelles called melanosomes to the actin cytoskeleton in melanocytes, and is required for visible pigmentation in the hair and skin. A mutation in this gene results in Griscelli syndrome type 3, which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eQuantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.\u003c\/li\u003e \u003cli\u003eIsoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.\u003c\/li\u003e \u003cli\u003eContext-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e compare relative target abundance and apparent size shifts (e.g., isoforms\/PTMs) across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e assess distribution across tissue compartments and compare staining patterns between groups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence \/ ICC:\u003c\/strong\u003e evaluate subcellular localization and co-localization with compartment markers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and compare shifts after stimulation or differentiation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAcross these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eApparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.\u003c\/li\u003e \u003cli\u003eSpecies reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.\u003c\/li\u003e \u003cli\u003eControl concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO\/KD, peptide competition, or independent assays) to support interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eFor antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProt entry for MLPH (Q9BV36) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/Q9BV36 - Ensembl gene summary for MLPH — Ensembl — https:\/\/www.ensembl.org - HGNC gene symbol report for MLPH — HGNC — https:\/\/www.genenames.org - Antibody validation concepts (general) — NIH\/NCBI resources — https:\/\/www.ncbi.nlm.nih.gov --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53068655001965,"sku":"A06081-3","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53069439762797,"sku":"A06081-3-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53069439795565,"sku":"A06081-3-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53069439828333,"sku":"A06081-3-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53069439861101,"sku":"A06081-3-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53069439893869,"sku":"A06081-3-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53069439926637,"sku":"A06081-3-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53069439959405,"sku":"A06081-3-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53069439992173,"sku":"A06081-3-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53069440024941,"sku":"A06081-3-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53069440057709,"sku":"A06081-3-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53069440090477,"sku":"A06081-3-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a06081-3-mlph-primary-antibodies-wb-testing-1.jpg?v=1772611750","url":"https:\/\/www.ebiohippo.com\/products\/anti-mlph-antibody-picoband-bha21006154","provider":"BioHippo","version":"1.0","type":"link"}