{"product_id":"anti-nfat2-nfatc1-antibody-picoband-bha21002297","title":"Anti-NFAT2\/NFATC1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-NFAT2\/NFATC1 Antibody Picoband® is an antibody for NFATC1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NFATC1 (nuclear factor of activated T cells 1); UniProt: O95644\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 120 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, IHC, Flow Cytometry, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-NFAT2\/NFATC1 Antibody Picoband® catalog # A00340-2.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e cellular localization: Nucleus. Cytoplasm., tissue context: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare NFATC1 levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Evaluate spatial distribution of NFATC1 in tissue sections, considering fixation and antigen retrieval effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify NFATC1-positive populations in single-cell suspensions with appropriate gating and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Nucleus. Cytoplasm.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e Adaptive Immunity,Cardiogenesis,Cardiovascular,2339,Epigenetics and Nuclear Signaling,Immunology,Non-CD,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,T Cells,Transcription,Transcription Factors\/Regulators\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for O95644: https:\/\/www.uniprot.org\/uniprot\/O95644 - Ensembl search (NFATC1): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NFATC1 - Human Protein Atlas search (NFATC1): https:\/\/www.proteinatlas.org\/search\/NFATC1 - PubMed search (NFATC1): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NFATC1 --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067708531053,"sku":"A00340-2","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067844682093,"sku":"A00340-2-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067844714861,"sku":"A00340-2-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067844747629,"sku":"A00340-2-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067844780397,"sku":"A00340-2-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067844813165,"sku":"A00340-2-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067844845933,"sku":"A00340-2-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067844878701,"sku":"A00340-2-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067844911469,"sku":"A00340-2-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067844944237,"sku":"A00340-2-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067844977005,"sku":"A00340-2-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067845009773,"sku":"A00340-2-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a00340-2-nfatc1-primary-antibodies-wb-testing-1.jpg?v=1772609607","url":"https:\/\/www.ebiohippo.com\/products\/anti-nfat2-nfatc1-antibody-picoband-bha21002297","provider":"BioHippo","version":"1.0","type":"link"}