{"product_id":"anti-ngl-1-lrrc4c-antibody-n49a-2-bha21406007","title":"Anti-NGL-1\/LRRC4C Antibody (N49A\/2)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against NGL-1\/LRRC4C (Leucine-rich repeat-containing protein 4C) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eNGL is a molecular target commonly interrogated by antibody-based methods to measure protein expression, localization and regulation across biological systems. Antibodies against NGL support studies ranging from baseline expression profiling to stimulus- or disease-associated changes in abundance and subcellular distribution. For pathway-level interpretation, pair NGL detection with appropriate controls and complementary readouts (e.g., genetic perturbation or orthogonal assays).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eSubcellular localization is target- and context-dependent; use cell-type appropriate controls and, where possible, genetic KO\/KD as specificity controls.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., IHC, WB) is indicated in the specifications; conditions should be optimized by titration.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of NGL-1\/LRRC4C, and a positive control sample known to express NGL-1\/LRRC4C.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036946588013,"sku":"HA983013-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037564821869,"sku":"HA983013-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037564854637,"sku":"HA983013-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HA983013-SDS-PAGE-1.jpg?v=1771746263","url":"https:\/\/www.ebiohippo.com\/products\/anti-ngl-1-lrrc4c-antibody-n49a-2-bha21406007","provider":"BioHippo","version":"1.0","type":"link"}