{"product_id":"anti-noxa1-antibody-picoband-bha21007171","title":"Anti-NOXA1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-NOXA1 Antibody Picoband® is an antibody targeting \u003cstrong\u003eNOXA1\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, Flow Cytometry, ELISA\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eHuman\u003c\/strong\u003e; observed MW: \u003cstrong\u003e60 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e51 kDa\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-NOXA1 Antibody catalog # PA1930. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NOXA1 — NADPH oxidase activator 1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 60 kDa; Calculated: 51 kDa\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e Functions as an activator of NOX1, a superoxide- producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB\/gp91phox and NOX3. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details (datasheet):\u003c\/strong\u003e Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Belongs to the NCF2\/NOXA1 family.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Associated Proteins,Cardiovascular,Cell Biology,Hypoxia,Metabolism,Organelle Proteins,Oxidative Stress,Pathways and Processes,Protein Trafficking,Redox Metabolism,Signal Transduction.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069802045805,"sku":"PA1930","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070259487085,"sku":"PA1930-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070259519853,"sku":"PA1930-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070259552621,"sku":"PA1930-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070259585389,"sku":"PA1930-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070259618157,"sku":"PA1930-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070259650925,"sku":"PA1930-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070259683693,"sku":"PA1930-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070259716461,"sku":"PA1930-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070259749229,"sku":"PA1930-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070259781997,"sku":"PA1930-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070259814765,"sku":"PA1930-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pa1930-noxa1-primary-antibodies-wb-testing-1.jpg?v=1772612599","url":"https:\/\/www.ebiohippo.com\/products\/anti-noxa1-antibody-picoband-bha21007171","provider":"BioHippo","version":"1.0","type":"link"}