{"product_id":"anti-nucleobindin-2-nucb2-antibody-picoband-bha21000777","title":"Anti-Nucleobindin 2\/NUCB2 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003eNucb2 (Nucleobindin-2)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-Nucleobindin 2\/NUCB2 Antibody Picoband® catalog # PB9876. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively. (reported region: V25-L106).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 55 kDa; calculated MW: 50304 MW\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Mouse,Rat\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e ELISA, IHC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eNucleobindin-2; Nucleobindin-2. Nucleobindin-2?is a?calcium-binding?EF-hand protein?that in humans is encoded by the?NUCB2?gene. By exon trapping and sequence analysis,?the?NUCB2?sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. Functional note: Calcium-binding protein. May have a role in calcium homeostasis. . Reported localization: Cytoplasm . Perikaryon . Endoplasmic reticulum . Golgi apparatus . Nucleus envelope . Membrane ; Peripheral membrane protein . Secreted . In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted. Expression\/tissue context: Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. .\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCalcium Binding Proteins: Researchers commonly examine how Nucb2 (Nucleobindin-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCalcium Signaling: Researchers commonly examine how Nucb2 (Nucleobindin-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eChromatin Binding Proteins: Researchers commonly examine how Nucb2 (Nucleobindin-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative Nucb2 (Nucleobindin-2) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of Nucb2 (Nucleobindin-2) across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for P81117: https:\/\/www.uniprot.org\/uniprotkb\/P81117\/entry - PubMed search (Nucb2): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Nucb2 - NCBI Gene search (Nucb2): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Nucb2 - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066837557613,"sku":"PB9876","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067169726829,"sku":"PB9876-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067169759597,"sku":"PB9876-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067169792365,"sku":"PB9876-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067169825133,"sku":"PB9876-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067169857901,"sku":"PB9876-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067169890669,"sku":"PB9876-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067169923437,"sku":"PB9876-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067169956205,"sku":"PB9876-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067169988973,"sku":"PB9876-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067170021741,"sku":"PB9876-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067170054509,"sku":"PB9876-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pb9876-1-WB-anti-nesfatin-1-picoband-antibody.jpg?v=1772608207","url":"https:\/\/www.ebiohippo.com\/products\/anti-nucleobindin-2-nucb2-antibody-picoband-bha21000777","provider":"BioHippo","version":"1.0","type":"link"}