{"product_id":"anti-oct4-pou5f1-antibody-picoband-bha21002300","title":"Anti-Oct4\/POU5F1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-Oct4\/POU5F1 Antibody Picoband® is an antibody for POU5F1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POU5F1 (POU class 5 homeobox 1); UniProt: Q01860\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 45 kDa, calculated 38,571 MW\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, IHC, Flow Cytometry, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-Oct4\/POU5F1 Antibody catalog # A00174-1.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e cellular localization: Nucleus. Cytoplasm., tissue context: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare POU5F1 levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Evaluate spatial distribution of POU5F1 in tissue sections, considering fixation and antigen retrieval effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify POU5F1-positive populations in single-cell suspensions with appropriate gating and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Nucleus. Cytoplasm.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e 2339,Epigenetics and Nuclear Signaling\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for Q01860: https:\/\/www.uniprot.org\/uniprot\/Q01860 - Ensembl search (POU5F1): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POU5F1 - Human Protein Atlas search (POU5F1): https:\/\/www.proteinatlas.org\/search\/POU5F1 - PubMed search (POU5F1): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POU5F1 --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067708858733,"sku":"A00174-1","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067843600749,"sku":"A00174-1-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067843633517,"sku":"A00174-1-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067843666285,"sku":"A00174-1-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067843699053,"sku":"A00174-1-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067843731821,"sku":"A00174-1-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067843764589,"sku":"A00174-1-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067843797357,"sku":"A00174-1-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067843830125,"sku":"A00174-1-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067843862893,"sku":"A00174-1-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067843895661,"sku":"A00174-1-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067843928429,"sku":"A00174-1-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a00174-1-pou5f1-primary-antibodies-wb-testing-1.jpg?v=1772609606","url":"https:\/\/www.ebiohippo.com\/products\/anti-oct4-pou5f1-antibody-picoband-bha21002300","provider":"BioHippo","version":"1.0","type":"link"}