{"product_id":"anti-pacsin3-antibody-bha21300552","title":"Anti-PACSIN3 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-PACSIN3 Antibody is an antibody targeting Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII (also reported as Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Intracellular.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Rat, human - identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Unconjugated (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003ePacsin 3 (also known as Syndapin 3) is a member of the highly conserved Pacsin protein sub-family which constitutes a branch of the larger F-BAR domain protein family.Pacsin 3, as well as other members of its family, is a cytoplasmic protein involved in receptor-mediated endocytosis, synaptic vesicle trafficking and biogenesis of different cellular organelles and is mostly, but not only, expressed in lung and muscle tissues. Pacsin 3 contains an N-terminal F-BAR domain and a C-terminal mono-Src homology 3 domain1.The C-terminal SH3 domain of Pacsin 3 interacts with the N-terminal proline-rich domain of TRPV4 at residues 132-144 and enhances the cell surface expression of TRPV4, probably through the inhibition of the endocytotic process2. The SH3 domain is also responsible for interactions with the protein dynamin and with N-WASP, a potent activator of the Arp2\/3 complex F-actin-nucleation machine3.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eComparing target expression across perturbations, genotypes, or treatment conditions.\u003c\/li\u003e   \u003cli\u003eInterpreting localization shifts alongside pathway or phenotypic readouts.\u003c\/li\u003e   \u003cli\u003eUsing orthogonal controls (KO\/KD, peptide competition, isotype concepts) to support conclusions.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-IP015.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-IP015; Negative control: BLP-IP015.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064832844141,"sku":"AIP-015-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064910438765,"sku":"AIP-015-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064910471533,"sku":"AIP-015-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064910504301,"sku":"AIP-015-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AIP-015_ept.gif?v=1772460994","url":"https:\/\/www.ebiohippo.com\/products\/anti-pacsin3-antibody-bha21300552","provider":"BioHippo","version":"1.0","type":"link"}