{"product_id":"anti-pbk-antibody-picoband-bha21000196","title":"Anti-PBK Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003ePBK (Lymphokine-activated killer T-cell-originated protein kinase)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-PBK Antibody Picoband® catalog # PB9310. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK. (reported region: N71-V322).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 40 kDa; calculated MW: 36085 MW\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human,Mouse,Rat\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e Flow Cytometry, IF, IHC, ICC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eLymphokine-activated killer T-cell-originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase. Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine\/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Functional note: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2\/M checkpoint during doxorubicin-induced DNA damage. . Reported localization: Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. Expression\/tissue context: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. .\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCancer: Researchers commonly examine how PBK (Lymphokine-activated killer T-cell-originated protein kinase) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCell Cycle: Researchers commonly examine how PBK (Lymphokine-activated killer T-cell-originated protein kinase) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCell Cycle Inhibitors: Researchers commonly examine how PBK (Lymphokine-activated killer T-cell-originated protein kinase) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative PBK (Lymphokine-activated killer T-cell-originated protein kinase) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of PBK (Lymphokine-activated killer T-cell-originated protein kinase) across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eIF\/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation\/permeabilization and epitope accessibility.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFamily \/ similarity context:\u003c\/strong\u003e Belongs to the protein kinase superfamily. STE Ser\/Thr protein kinase family. MAP kinase kinase subfamily.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for Q96KB5: https:\/\/www.uniprot.org\/uniprotkb\/Q96KB5\/entry - PubMed search (PBK): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PBK - NCBI Gene search (PBK): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PBK - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066818355565,"sku":"PB9310","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53066947428717,"sku":"PB9310-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53066947461485,"sku":"PB9310-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53066947494253,"sku":"PB9310-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53066947527021,"sku":"PB9310-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53066947559789,"sku":"PB9310-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53066947592557,"sku":"PB9310-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53066947625325,"sku":"PB9310-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53066947658093,"sku":"PB9310-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53066947690861,"sku":"PB9310-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53066947723629,"sku":"PB9310-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53066947756397,"sku":"PB9310-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pb9310-pbk-primary-antibodies-wb-testing-1.jpg?v=1772608110","url":"https:\/\/www.ebiohippo.com\/products\/anti-pbk-antibody-picoband-bha21000196","provider":"BioHippo","version":"1.0","type":"link"}