{"product_id":"anti-pdha1-polyclonal-antibody-bha21404600","title":"Anti-PDHA1 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003ePyruvate dehydrogenase E1 component subunit alpha is an oxidoreductase (dehydrogenase) involved in metabolic redox reactions, often using NAD(H) or NADP(H) cofactors. Dehydrogenases are widely assessed in metabolic reprogramming, mitochondrial function and tissue-specific energy metabolism studies.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eOften cytosolic or mitochondrial depending on enzyme class; consult known isoforms and compartmentalization for interpretation.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., ELISA, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Mouse, Rat).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of PDHA1, and a positive control sample known to express PDHA1.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eGenerated using an immunogen derived from E. coli - derived recombinant Human PDHA1 (Phe30-Ser390).. When interpreting results, note that epitope accessibility can vary with denaturation, fixation, or post‑translational modifications; empirical optimization is recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036900024685,"sku":"HY552014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037484507501,"sku":"HY552014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HY552014-abinscience-WB-1.jpg?v=1771745478","url":"https:\/\/www.ebiohippo.com\/products\/anti-pdha1-polyclonal-antibody-bha21404600","provider":"BioHippo","version":"1.0","type":"link"}