{"product_id":"anti-phkg1-polyclonal-antibody-bha21416507","title":"Anti-PHKG1 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-PHKG1 Polyclonal Antibody targets \u003cstrong\u003ePHKG1\u003c\/strong\u003e (Phosphorylase b kinase gamma catalytic chain). Frequently used across ELISA, IHC, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Cell Signaling research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Phosphorylase b kinase gamma catalytic chain, Phosphorylase kinase subunit gamma-1, Serine\/threonine-protein kinase PHKG1, skeletal muscle\/heart isoform, PHK-gamma-M, 2.7.11.19, 2.7.11.26, 2.7.11.1, PHKG.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003ePHKG1 (Phosphorylase b kinase gamma catalytic chain) encodes a kinase that regulates cellular processes through phosphorylation of protein substrates. Kinase activation and phosphorylation-dependent mobility shifts may influence Western blot banding and assay interpretation. Common alternative names and symbols used in the literature include: Phosphorylase kinase subunit gamma-1, Serine\/threonine-protein kinase PHKG1, skeletal muscle\/heart isoform, PHK-gamma-M, 2.7.11.19, 2.7.11.26, 2.7.11.1, PHKG. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a polyclonal reagent, this antibody can provide strong sensitivity through recognition of multiple epitopes on the target. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eAntigen \/ immunogen context\u003c\/h3\u003e\u003cp\u003eE. coli - derived recombinant human PHKG1 (Met1-Gln289).\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037204996461,"sku":"HB537014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037843906925,"sku":"HB537014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-phkg1-polyclonal-antibody-bha21416507","provider":"BioHippo","version":"1.0","type":"link"}