{"product_id":"anti-phospho-h2ax-ps139-antibody-a9-bha21408706","title":"Anti-Phospho-γ-H2AX (pS139) Antibody (A9)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Phospho-γ-H2AX (pS139) Antibody (A9) targets \u003cstrong\u003ePhospho-γ-H2AX (pS139)\u003c\/strong\u003e (Histone H2A.X). Frequently used across IHC, IP, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Gene Expression \u0026amp; Epigenetics research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Histone H2A.X, Histone H2AX, gamma H2A.X, H2a\/x, H2AFX, H2AX.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003ePhospho-γ-H2AX (pS139) (Histone H2A.X) is a modification-specific target used to track protein phosphorylation state rather than total protein abundance. Phospho-epitopes are often stimulation- and fixation-sensitive; rapid sample processing and inclusion of phosphatase inhibitors can be important for preserving signal. Phospho-H2AX (γ-H2AX) is widely used as a marker of DNA damage response signaling and double-strand break–associated chromatin changes. Common alternative names and symbols used in the literature include: Histone H2AX, gamma H2A.X, H2a\/x, H2AFX, H2AX. Based on the annotated name\/class, the target is most often associated with nucleus localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037036667245,"sku":"HB952023-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037747110253,"sku":"HB952023-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037747143021,"sku":"HB952023-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB952023-SDS-PAGE-1.jpg?v=1771747956","url":"https:\/\/www.ebiohippo.com\/products\/anti-phospho-h2ax-ps139-antibody-a9-bha21408706","provider":"BioHippo","version":"1.0","type":"link"}