{"product_id":"anti-phospho-mlkl-s345-rabbit-monoclonal-antibody-bha21010255","title":"Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody","description":"\u003cp\u003eBoster Bio Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody catalog # P00535. Tested in WB, IP applications. This antibody reacts with Mouse.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGene\u003c\/strong\u003e: RAF proto-oncogene serine\/threonine-protein kinase | \u003cstrong\u003eTarget\u003c\/strong\u003e: Mlkl | \u003cstrong\u003eFunction\u003c\/strong\u003e: Serine\/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK\/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1\/MEK1 and MAP2K2\/MEK2) and the extracellular signal-regulated kinases (MAPK3\/ERK1 and MAPK1\/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD\/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2\/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5\/ASK1 and STK3\/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD\/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2\/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. . | \u003cstrong\u003eResearch areas\u003c\/strong\u003e: Apoptosis,Cancer,Cell Biology,Cell Death,Metabolism,Metabolism Processes,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Oncoproteins,Oncoproteins\/Suppressors,Pathways and Processes,Protein Phosphorylation,Ser\/Thr Kinases,Signal Transduction\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e: Rabbit | \u003cstrong\u003eClonality\/Clone\u003c\/strong\u003e: Monoclonal \/ Clone: 27M64 | \u003cstrong\u003eIsotype\u003c\/strong\u003e: IgG | \u003cstrong\u003eReactivity\u003c\/strong\u003e: Mouse | \u003cstrong\u003eImmunogen\u003c\/strong\u003e: A synthesized peptide derived from human Phospho-MLKL (S345) | \u003cstrong\u003eConcentration\u003c\/strong\u003e: 0.5mg\/ml | \u003cstrong\u003ePurification\u003c\/strong\u003e: Affinity-chromatography\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e: WB, IP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eObserved MW\u003c\/strong\u003e: 54 kDa | \u003cstrong\u003eCalculated MW\u003c\/strong\u003e: 73052 MW\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization\u003c\/strong\u003e: Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details\u003c\/strong\u003e: In skeletal muscle, isoform 1 is more abundant than isoform 2. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePhospho site(s)\u003c\/strong\u003e: S345\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStatus\u003c\/strong\u003e: Active\u003c\/p\u003e","brand":"Boster Bio","offers":[{"title":"100 uL\/vial \/ Unconjugated","offer_id":53072119464301,"sku":"P00535","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/p00535-wb.jpg?v=1772621948","url":"https:\/\/www.ebiohippo.com\/products\/anti-phospho-mlkl-s345-rabbit-monoclonal-antibody-bha21010255","provider":"BioHippo","version":"1.0","type":"link"}