{"product_id":"anti-phospho-smad2-pt8-antibody-saa0289-bha21408724","title":"Anti-Phospho-Smad2 (pT8) Antibody (SAA0289)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-Phospho-Smad2 (pT8) Antibody (SAA0289) targets \u003cstrong\u003ePhospho-Smad2 (pT8)\u003c\/strong\u003e (Mothers against decapentaplegic homolog 2). Frequently used across IHC, IP, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Cell Signaling research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad-related protein 2, SMAD family member 2, MAD homolog 2, SMAD 2, JV18-1, hMAD-2.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003ePhospho-Smad2 (pT8) (Mothers against decapentaplegic homolog 2) is a modification-specific target used to track protein phosphorylation state rather than total protein abundance. Phospho-epitopes are often stimulation- and fixation-sensitive; rapid sample processing and inclusion of phosphatase inhibitors can be important for preserving signal. Common alternative names and symbols used in the literature include: Mothers against DPP homolog 2, Mad-related protein 2, SMAD family member 2, MAD homolog 2, SMAD 2, JV18-1, hMAD-2. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037037715821,"sku":"HS710023-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037748289901,"sku":"HS710023-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037748322669,"sku":"HS710023-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HS710023-SDS-PAGE-1.jpg?v=1771747954","url":"https:\/\/www.ebiohippo.com\/products\/anti-phospho-smad2-pt8-antibody-saa0289-bha21408724","provider":"BioHippo","version":"1.0","type":"link"}