{"product_id":"anti-pkc-theta-prkcq-antibody-picoband-bha21002857","title":"Anti-PKC theta\/PRKCQ Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-PKC theta\/PRKCQ Antibody Picoband® is an antibody for PRKCQ detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRKCQ (cannabinoid receptor 1); UniProt: Q04759\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 78 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, IHC, Flow Cytometry, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-PKC theta\/PRKCQ Antibody Picoband® catalog # A01293-3.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta (9)-tetrahydrocannabinol (THC) (PubMed:15620723, PubMed:27768894, PubMed:27851727). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP (PubMed:1718258, PubMed:21895628, PubMed:27768894). In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses. At high doses, CNR1 signal transduction involves G-protein alpha-i protein activation and subsequent inhibition of mitochondrial soluble adenylate cyclase, decrease in cyclic AMP concentration, inhibition of protein kinase A (PKA)-dependent phosphorylation of specific subunits of the mitochondrial electron transport system, including NDUFS2. In the hypothalamus, inhibits leptin-induced reactive oxygen species (ROS) formation and mediates cannabinoid-induced increase in SREBF1 and FASN gene expression. In response to cannabinoids, drives the release of orexigenic beta-endorphin, but not that of melanocyte-stimulating hormone alpha\/alpha-MSH, from hypothalamic POMC neurons, hence promoting food intake. In the hippocampus, regulates cellular respiration and energy production in response to cannabinoids. Involved in cannabinoid-dependent depolarization-induced suppression of inhibition (DSI), a process in which depolarization of CA1 postsynaptic pyramidal neurons mobilizes eCBs, which retrogradely activate presynaptic CB1 receptors, transiently decreasing GABAergic inhibitory neurotransmission. Also reduces excitatory synaptic transmission (By similarity). In superior cervical ganglions and cerebral vascular smooth muscle cells, inhibits voltage-gated Ca (2+) channels in a constitutive, as well as agonist-dependent manner (PubMed:17895407). In cerebral vascular smooth muscle cells, cannabinoid-induced inhibition of voltage-gated Ca (2+) channels leads to vasodilation and decreased vascular tone (By similarity). Induces leptin production in adipocytes and reduces LRP2-mediated leptin clearance in the kidney, hence participating in hyperleptinemia. In adipose tissue, CNR1 signaling leads to increased expression of SREBF1, ACACA and FASN genes (By similarity). In the liver, activation by endocannabinoids leads to increased de novo lipogenesis and reduced fatty acid catabolism, associated with increased expression of SREBF1\/SREBP-1, GCK, ACACA, ACACB and FASN genes. May also affect de novo cholesterol synthesis and HDL-cholesteryl ether uptake. Peripherally modulates energy metabolism (By similarity). In high carbohydrate diet- induced obesity, may decrease the expression of mitochondrial dihydrolipoyl dehydrogenase\/DLD in striated muscles, as well as that of selected glucose\/ pyruvate metabolic enzymes, hence affecting energy expenditure through mitochondrial metabolism (By similarity). In response to cannabinoid anandamide, elicits a proinflammatory response in macrophages, which involves NLRP3 inflammasome activation and IL1B and IL18 secretion (By similarity). In macrophages infiltrating pancreatic islets, this process may participate in the progression of type-2 diabetes and associated loss of pancreatic beta-cells (PubMed:23955712).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e cellular localization: Cell membrane., tissue context: Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare PRKCQ levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Evaluate spatial distribution of PRKCQ in tissue sections, considering fixation and antigen retrieval effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify PRKCQ-positive populations in single-cell suspensions with appropriate gating and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e Protein kinase C theta (PKC-θ) is an enzyme that in humans is encoded by the PRKCQ gene. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Cell membrane.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e Atherosclerosis,Cancer,Cardiovascular,Cholesterol Metabolism,Drug Metabolism,Heart Disease,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids\/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neuroscience,Neurotransmission,Pathways and Processes,Receptors \/ Channels,Signal Transduction\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for Q04759: https:\/\/www.uniprot.org\/uniprot\/Q04759 - Ensembl search (PRKCQ): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRKCQ - Human Protein Atlas search (PRKCQ): https:\/\/www.proteinatlas.org\/search\/PRKCQ - PubMed search (PRKCQ): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRKCQ --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067728060781,"sku":"A01293-3","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53068051448173,"sku":"A01293-3-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53068051480941,"sku":"A01293-3-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53068051513709,"sku":"A01293-3-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53068051546477,"sku":"A01293-3-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53068051579245,"sku":"A01293-3-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53068051612013,"sku":"A01293-3-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53068051644781,"sku":"A01293-3-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53068051677549,"sku":"A01293-3-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53068051710317,"sku":"A01293-3-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53068051743085,"sku":"A01293-3-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53068051775853,"sku":"A01293-3-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01293-3-prkcq-primary-antibodies-wb-testing-1.jpg?v=1772609719","url":"https:\/\/www.ebiohippo.com\/products\/anti-pkc-theta-prkcq-antibody-picoband-bha21002857","provider":"BioHippo","version":"1.0","type":"link"}