{"product_id":"anti-prc1-antibody-picoband-bha21000714","title":"Anti-PRC1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003ePRC1 (Protein regulator of cytokinesis 1)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-PRC1 Antibody Picoband® catalog # PB9814. Tested in Flow Cytometry, IP, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1. (reported region: M1-E233).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 72 kDa; calculated MW: 72 kDa\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human,Mouse,Rat\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e Flow Cytometry, IP, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eProtein regulator of cytokinesis 1; Protein regulator of cytokinesis 1. Protein Regulator of cytokinesis 1 (PRC1) is a protein that in humans is encoded by the PRC1 gene and is involved in cytokinesis. This gene is mapped to chromosome 15q26.1 based on an alignment of the PRC1 sequence with the genomic sequence. PRC1 protein is expressed at relatively high levels during S and G2\/M phases of the cell cycle before dropping dramatically after mitotic exit and entrance into G1 phase. It is located in the nucleus during interphase, becomes associated with the mitotic spindle in a highly dynamic manner during anaphase, and localizes to the cell midbody during cytokinesis. And this protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. Functional note: Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436). . Reported localization: Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody . Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. . Expression\/tissue context: Overexpressed in bladder cancer cells (PubMed:17409436). .\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCell Cycle: Researchers commonly examine how PRC1 (Protein regulator of cytokinesis 1) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCell Division: Researchers commonly examine how PRC1 (Protein regulator of cytokinesis 1) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCytokinesis: Researchers commonly examine how PRC1 (Protein regulator of cytokinesis 1) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative PRC1 (Protein regulator of cytokinesis 1) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for O43663: https:\/\/www.uniprot.org\/uniprotkb\/O43663\/entry - PubMed search (PRC1): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRC1 - NCBI Gene search (PRC1): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRC1 - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066835853677,"sku":"PB9814","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067145937261,"sku":"PB9814-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067145970029,"sku":"PB9814-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067146002797,"sku":"PB9814-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067146035565,"sku":"PB9814-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067146068333,"sku":"PB9814-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067146101101,"sku":"PB9814-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067146133869,"sku":"PB9814-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067146166637,"sku":"PB9814-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067146199405,"sku":"PB9814-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067146232173,"sku":"PB9814-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067146264941,"sku":"PB9814-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pb9814-prc1-primary-antibodies-wb-testing-1.jpg?v=1772608200","url":"https:\/\/www.ebiohippo.com\/products\/anti-prc1-antibody-picoband-bha21000714","provider":"BioHippo","version":"1.0","type":"link"}