{"product_id":"anti-prospero-homeobox-protein-1-prox1-antibody-picoband-bha21007411","title":"Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-Prospero homeobox protein 1 PROX1 Antibody Picoband® is an antibody targeting \u003cstrong\u003ePROX1\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, Flow Cytometry, ELISA\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eRat,Mouse,Human\u003c\/strong\u003e; observed MW: \u003cstrong\u003e55 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e83203 MW\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PROX1 — Prospero homeobox protein 1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Rat,Mouse,Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 55 kDa; Calculated: 83203 MW\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA\/G-target genes including core clock components: ARNTL\/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Nucleus . RORG promotes its nuclear localization. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details (datasheet):\u003c\/strong\u003e Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Belongs to the Prospero homeobox family.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Cardiogenesis,Cardiovascular,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Neural Signal Transduction,Neurology Process,Neuroscience,Transcription,Transcription Factors\/Regulators.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069809582445,"sku":"PA2229","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070346781037,"sku":"PA2229-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070346813805,"sku":"PA2229-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070346846573,"sku":"PA2229-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070346879341,"sku":"PA2229-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070346912109,"sku":"PA2229-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070346944877,"sku":"PA2229-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070346977645,"sku":"PA2229-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070347010413,"sku":"PA2229-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070347043181,"sku":"PA2229-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070347075949,"sku":"PA2229-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070347108717,"sku":"PA2229-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/blank_cc403dbd-3f32-4d11-a074-3f4d7db1ad2e.png?v=1772558524","url":"https:\/\/www.ebiohippo.com\/products\/anti-prospero-homeobox-protein-1-prox1-antibody-picoband-bha21007411","provider":"BioHippo","version":"1.0","type":"link"}