{"product_id":"anti-prrsv-gp2-polyclonal-antibody-bha21422870","title":"Anti-PRRSV GP2 Polyclonal Antibody","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePrimary Antibody · Rabbit Polyclonal\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAnti-PRRSV GP2 Polyclonal Antibody\u003c\/strong\u003e is an affinity-purified rabbit polyclonal antibody raised against the GP2 (GP2a) minor envelope glycoprotein of \u003cstrong\u003eporcine reproductive and respiratory syndrome virus 2 (PRRSV2)\u003c\/strong\u003e, encoded by ORF2. It is supplied unconjugated in liquid form and is validated by the manufacturer for ELISA, immunohistochemistry and Western blot.\u003c\/p\u003e\n\u003cp\u003ePRRSV is an enveloped, positive-sense single-stranded RNA arterivirus and one of the most economically significant swine pathogens worldwide. Its minor envelope glycoproteins GP2, GP3 and GP4 assemble into a heterotrimeric complex that engages the macrophage receptor CD163 during entry, which makes GP2 a recurring subject of entry-mechanism, receptor-binding, seroprevalence and vaccine-antigen studies.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003ctd\u003eRecommended dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eELISA\u003c\/th\u003e\n\u003ctd\u003e1:5,000 – 1:20,000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunohistochemistry (IHC)\u003c\/th\u003e\n\u003ctd\u003e1:50 – 1:500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eWestern blot (WB)\u003c\/th\u003e\n\u003ctd\u003e1:500 – 1:2,000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eDilutions are manufacturer starting points. Titrate for each sample type, fixation method and detection system, and include a known positive antigen alongside a target-negative control.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies reactivity\u003c\/th\u003e\n\u003ctd\u003ePorcine reproductive and respiratory syndrome virus 2 (PRRSV2)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eGP2 glycosylated envelope protein, ORF2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen\u003c\/th\u003e\n\u003ctd\u003eE. coli - derived recombinant PRRSV GP2 (Ser41-Ser216).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003ePurified by antigen affinity column.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003e0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eABO93140.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and storage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eImmunogen \u0026amp; Antigen\u003c\/h2\u003e\n\u003cp\u003eThe antiserum was raised against an \u003cem\u003eE. coli\u003c\/em\u003e-derived recombinant PRRSV GP2 fragment spanning \u003cstrong\u003eSer41–Ser216\u003c\/strong\u003e and then purified on an antigen affinity column, so the antibody pool is enriched for antibodies recognising that region of GP2.\u003c\/p\u003e\n\u003cp\u003eManufacturer Western blot data show a single reactive band against 100 ng of recombinant GP2 antigen. Because the immunogen is a bacterially expressed fragment, the observed size reflects the recombinant antigen rather than full-length, natively glycosylated GP2 — expect a different apparent molecular weight when probing infected-cell lysates or virions.\u003c\/p\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eManufacturer-stated reactivity is limited to \u003cstrong\u003ePRRSV2\u003c\/strong\u003e (the North American genotype). Cross-reactivity with PRRSV-1 (European genotype) isolates, with other arteriviruses, or with uninfected porcine tissue is not claimed and should be verified in-house before use in a comparative study.\u003c\/p\u003e\n\u003cp\u003eEpitopes presented by a non-glycosylated bacterial fragment may be partially masked on native, heavily glycosylated GP2. Where staining or blotting signal is weak, compare reducing and non-reducing conditions and consider an antigen-retrieval optimisation series for IHC.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eThe formulation contains \u003cstrong\u003e0.05% Proclin 300\u003c\/strong\u003e, a preservative that is harmful on contact — handle with gloves and avoid skin and eye exposure. The 50% glycerol content means the product does not freeze solid at -20 °C; aliquot before long-term storage and avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003ca href=\"\/products\/recombinant-prrsv-gp2-protein-c-fc-bhp21402876\"\u003eRecombinant PRRSV GP2 Protein, C-Fc\u003c\/a\u003e — matching recombinant antigen for standard curves, positive controls and blocking experiments.\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":53816464638317,"sku":"VK459014-50UG","price":268.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53816498782573,"sku":"VK459014-100UG","price":378.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VK459014-WB-1.jpg?v=1787770548","url":"https:\/\/www.ebiohippo.com\/products\/anti-prrsv-gp2-polyclonal-antibody-bha21422870","provider":"BioHippo","version":"1.0","type":"link"}